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KMID : 1098420070150060444
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 6 p.444 ~ p.450
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Extract of Cedrela sinensis Leaves Protects Neuronal Cell Damage Induced by Hydrogen Peroxide in Cultured Rat Neurons
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Lee Soon-Bok
Kim Ju-Yeon
Ju Hyun-Soo
Bae Ki-Hwan
Cho Soon-Ock
Seong Yeon-Hee
Ban Ju-Yeon
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Abstract
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Dried leaves from Cedrela sinensis A. Juss. (CS), have been observed to possess various pharmacological activity and contain various antioxidant constituents. The protective effect of ethanol extract of CS on hydrogen peroxide neurotoxicity was examined using primary cultured rat cortical neurons in the present study. Exposure of cultured neurons to 100 caused a significant neuronal death as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. The addition of CS, over a concentration range of 10 to , concentration-dependently prevented the neuronal apoptotic death. CS significantly inhibited elevation of the cytosolic concentration , which was measured by a fluorescent dye, Fluo-4 AM. CS (30 and inhibited glutamate release and generation of reactive oxygen species (ROS) induced by . These results suggest that CS may mitigate the neurotoxiciy by interfering with the increase of , and then inhibiting glutamate release and generation of ROS in cultured neurons.
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KEYWORD
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Cedrela sinensis, neurotoxicity, hydrogen peroxide, neuroprotection, cortical neurons
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